Enzymes Unit - Ch 6
Energy and Enzymes - Objectives
1. explain the role of catabolic and anabolic pathways in the energy exchanges of cellular metabolism and distinguish between kinetic and potential energy.
2. Evaluate how life requires a highly ordered system and order in life is maintained by constant free energy input into the system.
3. explain how loss of order or free energy flow results in death.
4. describe how increased entropy in living systems must be offset by biological processes that maintain or increase order.
5. illustrate how living systems do not violate the second law of thermodynamics, which states that entropy increases over time.
6. explain how energy input must exceed free energy lost to entropy to maintain order and power
cellular processes in living systems.
7. define how energetically favorable exergonic reactions, such as ATP→ADP, that have
a negative change in free energy can be used to maintain or increase order in a system
by being coupled with reactions that have a positive free energy change.
8. distinguish between exergonic and endergonic reactions.
9. explain and correctly construct potential energy diagrams for endothermic and exothermic reactions.
10. draw the structure of an ATP, describe the ATP cycle as related to energy released and gained, and list/describe the functions of ATP.
12. describe the basic structure of an enzyme.
13. define the terms substrate and active site.
14. describe the lock and key and induced fit hypotheses of enzyme function..
15. explain what allosteric effects are in relation to enzyme function.
16. recognize that most enzymes function best at a neutral pH and explain why this is so.
17. recognize the effects of temperature on the function of most enzymes and relate these effects to the kinetic molecular.theory.
18. define the term denaturization and list three factors which can cause this to occur
19. recognize the effects when a fixed amount of enzyme is placed with an excess of substrate and explain why these effects occur.
20. recognize the effects when a fixed amount of substrate is placed with an excess of enzyme and explain why these effects occur.
21. describe the role of coenzymes and cofactors in relation to the structure and function of enzymes.
22. explain how enzyme activity can be regulated by environmental conditions, cofactors, enzyme inhibitors, and allosteric regulators.
23. explain how metabolic pathways are regulated by allosteric effects.
Life Requires Free Energy
Video – Life Requires Free Energy (post 5 main points from video)http://www.bozemanscience.com/science-videos/2011/9/14/012-life-requires-free-energy.html
What is Gibbs Free Energy? (post 3 main points from video)
http://www.bozemanscience.com/science-videos/2011/9/14/gibbs-free-energy.html
Enzymes
PPT
Enzymes – This is important to review and study!
http://www.explorebiology.com/pptAP/2008/METABOLISM/31Ch08enzymes2008.ppt
WS
Energy and Enzymes – Guided ReadingAll you ever wanted to know about enzymes - Reading Sheet
Enzymes at Work WS
Lab
Denaturation of Protein Lab
Catalase Lab – 2007
Blog Project
Enzymes WS and Blog PostVideos
Thermodynamics and Enzymes (post 5 main points from video)
http://www.bozemanscience.com/science-videos/2010/9/5/enzymes-the-working-cell.html
Enzymes (post 5 main points from video)
http://www.bozemanscience.com/science-videos/2011/11/30/048-enzymes.html Enzymes – Bronxville – Better info on enzyme regulation (post 5 main points from video)
http://www.youtube.com/watch?v=CXgrbEN956k&feature=mfu_in_order&list=ULAnimations
Hydrolysis of Sucrose (post 3 main points from video)
http://highered.mcgraw-hill.com/sites/9834092339/student_view0/chapter6/enzyme_action_and_the_hydrolysis_of_sucrose.htmlBiochemical Pathways (post 3 main points from video)
http://highered.mcgraw-hill.com/sites/9834092339/student_view0/chapter6/a_biochemical_pathway.htmlFeedback Inhibition (post 3 main points from video)
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120070/bio10.swf::Feedback%20Inhibition%20of%20Biochemical%20Pathways